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NET 4.0, even though they are addressable if. ::SecurityProtocol = ::SecurityProtocol -bor 3072 # installed (.NET 4.5 is an in-place upgrade). # We use this variable for future REST calls. # If required, add the repository access credential here $NugetRepositoryUrl = "INTERNAL REPO URL" # Should be similar to what you see when you browse Your internal repository url (the main one).

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# This url should result in an immediate download when you navigate to it # $RequestArguments.Credential = $NugetRepositor圜redential # ("password" | ConvertTo-SecureString -AsPlainText -Force)

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# If using CCM to manage Chocolatey, add the following: $ChocolateyDownloadUrl = "$($NugetRepositoryUrl.TrimEnd('/'))/package/chocolatey.1.1.0.nupkg" #ALTERNATIVE TO SERIAL CLONER DOWNLOAD#

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Zip to the filename to handle archive cmdlet limitations # Ensure Chocolatey is installed from your internal repository # $Chocolate圜entralManagementServiceSalt = "servicesalt" # $Chocolate圜entralManagementClientSalt = "clientsalt" # $Chocolate圜entralManagementUrl = " # ii. If (-not (Get-Command choco.The CloneMiner cDNA Library Construction Kit is designed to construct high-quality cDNA libraries without the use of traditional restriction enzyme cloning methods. This novel technology combines the performance of SuperScript II Reverse Transcriptase with the Gateway Technology. Single-stranded mRNA is converted into double stranded cDNA containing attB sequences on each end. Through site-specific recombination, attB-flanked cDNA is cloned directly into an attP-containing donor vector without the use of restriction digestion or ligation. The resulting Gateway entry cDNA library can be screened with a probe to identify a specific entry clone.

Alternatively, the entire entry cDNA library can be shuttled into a Gateway destination vector to generate an expression library.įeatures of the CloneMiner cDNA Library Construction Kit include: This clone can be transferred into the Gateway destination vector of choice for gene expression and functional analysis.

SuperScript II reverse transcriptase for efficient conversion of mRNA into cDNA.

Biotin-attB2-Oligo(dT) Primer for poly(A) mRNA binding and incorporation of the attB2 sequence to the 3' end of cDNA.

attB1 Adapter for ligation of the attB1 sequence to the 5' end of double-stranded cDNA.

attP-containing vector (pDONR 222) for recombination with attB-flanked cDNA to produce an entry library through the Gateway BP recombination reactionĪdvantages of the CloneMiner cDNA Library Construction Kit.

Using CloneMiner cDNA Library Construction Kit offers the following advantages:.

Produces high yields of quality, double-stranded cDNA.Eliminates use of restriction enzyme digestion and ligation allowing cloning of undigested cDNA.#ALTERNATIVE TO SERIAL CLONER DOWNLOAD#.#ALTERNATIVE TO SERIAL CLONER SOFTWARE#.